milliplex sars-cov-2 antigen panel kits  (Merck & Co)

Journal: Viruses

Article Title: Distinguishing Incubation and Acute Disease Stages of Mild-to-Moderate COVID-19

doi: 10.3390/v14020203

Figure Lengend Snippet: Kinetics of plasmablast proportions and SARS-CoV-2 specific immunoglobulin enrichment delineated disease stages during mild-to-moderate COVID-19. ( A ) Representative pseudocolor plots of CD38 and CD27 expression data of CD19 + CD45RA + B lymphocytes showed the disease stage-dependent increase of plasmablast proportions (rectangular gate). ( B ) Top left: Quantitative data of plasmablast abundances at different disease stage. Top right, bottom left and bottom right: Semi-quantitative data of SARS-CoV-2 specific IgM, IgG and IgA titers in plasma samples. The data for anti-RBD, -S1, -S2 and –N were combined for IgM and IgG, respectively.* p < 0.05; ** p < 0.01; *** p < 0.001; Tukey-Kramer test for plasmablasts, Kruskal-Wallis test with Dunn’s correction for multiple comparisons for Antibody isotypes. MFI: Median fluorescence intensity. A 450 : Absorbance at 450 nm.

Article Snippet: For the semi-quantitative analyses of IgM and IgG specific for the nucleocapsid (N) or the spike protein subunits S1, S2 and receptor binding domain (RBD), we used the respective MILLIPLEX SARS-CoV-2 antigen panel kits (Merck) to the manufacturer’s instructions.

Techniques: Expressing, Fluorescence

Journal: medRxiv

Article Title: Distinguishing Incubation and Acute Disease Stages of Mild-to-Moderate COVID-19

doi: 10.1101/2021.09.07.21263200

Figure Lengend Snippet: ( A ) Representative pseudocolor plots of CD38 and CD27 expression data of CD19 + CD45RA + B lymphocytes showed the disease stage-dependent increase of plasmablast proportions (rectangular gate). ( B ) Top left: Quantitative data of plasmablast abundances at different disease stage. Top right, bottom left and bottom right: Semi-quantitative data of SARS-CoV-2 specific IgM, IgG and IgG titers in plasma samples. **p < 0.01; ***p < 0.001; Tukey-Kramer test for plasmablasts, Kruskal-Wallis test with Dunn’s correction for multiple comparisons for Antibody isotypes. MFI: Median fluorescence intensity. A 450 : Absorbance at 450 nm.

Article Snippet: For the semi-quantitative analyses of IgM and IgG specific for the nucleocapsid (N) or the spike protein subunits S1, S2 and receptor binding domain (RBD), we used the respective MILLIPLEX ® SARS-CoV-2 antigen panel kits (Merck) to the manufacturer’s instructions.

Techniques: Expressing, Fluorescence